BROMOSULFALEIN Property

solubility 

DMF: 5 mg/ml; DMSO: 5 mg/ml; Ethanol: slightly soluble; PBS (pH 7.2): 1 mg/ml

form 

Powder

Water Solubility 

Soluble in water.

Sensitive 

Moisture Sensitive

Merck 

14,8954

Stability:

Stable. Incompatible with strong oxidizing agents. May be air or moisture sensitive.

EPA Substance Registry System

Benzenesulfonic acid, 3,3'-(4,5,6,7-tetrabromo-3-oxo-1(3H)-isobenzofuranylidene)bis[6-hydroxy-, disodium salt (71-67-0)

Safety

Hazard Codes 

Xn

Risk Statements 

42/43

Safety Statements 

22-36/37

WGK Germany 

3

RTECS 

SM8395000

TSCA 

Yes

Hazardous Substances Data

71-67-0(Hazardous Substances Data)

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H317May cause an allergic skin reaction

H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P272Contaminated work clothing should not be allowed out of the workplace.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P285In case of inadequate ventilation wear respiratory protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P304+P341IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

P321Specific treatment (see … on this label).

P333+P313IF SKIN irritation or rash occurs: Get medical advice/attention.

P342+P311IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.

P363Wash contaminated clothing before reuse.

P501Dispose of contents/container to..…

BROMOSULFALEIN Chemical Properties,Usage,Production

Chemical Properties

white crystalline solid

Uses

Sulfobromophthalein sodium salt hydrate is a reagent for the quantitative determination of proteins, a dye for liver diagnostics in vitro.

Definition

ChEBI: An organic sodium salt that is the disodium salt of bromosulfophthalein.

Purification Methods

Purify it by TLC on silica Gel G (Merck 250a particle size) in two solvent systems (BuOH/AcOH/H2O 30:7.5:12.5 v/v, and BuOH/propionic acid/H2O 30:20:7.5 v/v). When the solvent reaches a height of ~10cm, the plate is removed, dried in air and developed with NH3 vapour giving blue-coloured spots. Also, the dye can be chromatographed on MN Silica Gel with t-BuOH/H2O/n-BuOH (32:10:5 v/v) as eluent and visualised with a dilute KOH (or NaOH if the Na salt is required) spray. The product corresponding to bromosulfalein is scraped off and eluted with H2O, filtered and evaporated to dryness in a vacuum. It was dissolved in H2O and filtered through Sephadex G-25 and evaporated to dryness. [UV and IR identification: Barbier & DeVeerdt J Pharm Sci 57 819 1968, NMR: Kato et al. Chem Pharm Bull Jpn 20 581 1972, McGuire Anal Biochem 83 75 1977, Beilstein 18/9 V 461.]